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recombinant human fgfr1 iiib  (R&D Systems)


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    R&D Systems recombinant human fgfr1 iiib
    Recombinant Human Fgfr1 Iiib, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human fgfr1 iiib/product/R&D Systems
    Average 90 stars, based on 4 article reviews
    recombinant human fgfr1 iiib - by Bioz Stars, 2026-02
    90/100 stars

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    recombinant human fgfr1 iiib  (R&D Systems)
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    Recombinant Human Fgfr1 Iiib, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    recombinant human fgfr1α iiib  (R&D Systems)
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    ADAM9 is required for the entry phase of EMCV infection. (A) EMCV or CV-B3 were pretreated with the indicated concentrations of soluble ADAM9 protein or 100 μg/ml soluble <t>FGFR1α/FGFR1β</t> proteins for 1 h at 37°C and used to infect HAP1 cells. Capsid proteins (EMCV) or 3A protein (CV-B3) and nuclei (blue) were stained at 7 h postinfection. (B) HAP1 cells were pretreated with the indicated concentrations of antibody targeting ADAM9 and infected with virus for 7 h, followed by staining as described above for panel A. Representative confocal micrographs are shown in panels A, B, and C. Values (percentages) on the micrographs are mean ± SEM values for 3 or 4 (A) or 4 (B) technical replicates, normalized to the value for mock treatment. (C) WT, ADAM9 KO , or ADAM9 KO HAP1 cells overexpressing mouse ADAM9 mutant E348A (mADAM9) were incubated with EMCV or CV-B3 on ice, followed by qPCR analysis of bound virus. The values are means plus standard deviations (SD) (error bars) for three biological replicates. Experiments were conducted twice with similar results.
    Recombinant Human Fgfr1α Iiib, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human fgfr1 iiib  (R&D Systems)
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    ADAM9 is required for the entry phase of EMCV infection. (A) EMCV or CV-B3 were pretreated with the indicated concentrations of soluble ADAM9 protein or 100 μg/ml soluble <t>FGFR1α/FGFR1β</t> proteins for 1 h at 37°C and used to infect HAP1 cells. Capsid proteins (EMCV) or 3A protein (CV-B3) and nuclei (blue) were stained at 7 h postinfection. (B) HAP1 cells were pretreated with the indicated concentrations of antibody targeting ADAM9 and infected with virus for 7 h, followed by staining as described above for panel A. Representative confocal micrographs are shown in panels A, B, and C. Values (percentages) on the micrographs are mean ± SEM values for 3 or 4 (A) or 4 (B) technical replicates, normalized to the value for mock treatment. (C) WT, ADAM9 KO , or ADAM9 KO HAP1 cells overexpressing mouse ADAM9 mutant E348A (mADAM9) were incubated with EMCV or CV-B3 on ice, followed by qPCR analysis of bound virus. The values are means plus standard deviations (SD) (error bars) for three biological replicates. Experiments were conducted twice with similar results.
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    rfgfr1b  (R&D Systems)
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    ADAM9 is required for the entry phase of EMCV infection. (A) EMCV or CV-B3 were pretreated with the indicated concentrations of soluble ADAM9 protein or 100 μg/ml soluble <t>FGFR1α/FGFR1β</t> proteins for 1 h at 37°C and used to infect HAP1 cells. Capsid proteins (EMCV) or 3A protein (CV-B3) and nuclei (blue) were stained at 7 h postinfection. (B) HAP1 cells were pretreated with the indicated concentrations of antibody targeting ADAM9 and infected with virus for 7 h, followed by staining as described above for panel A. Representative confocal micrographs are shown in panels A, B, and C. Values (percentages) on the micrographs are mean ± SEM values for 3 or 4 (A) or 4 (B) technical replicates, normalized to the value for mock treatment. (C) WT, ADAM9 KO , or ADAM9 KO HAP1 cells overexpressing mouse ADAM9 mutant E348A (mADAM9) were incubated with EMCV or CV-B3 on ice, followed by qPCR analysis of bound virus. The values are means plus standard deviations (SD) (error bars) for three biological replicates. Experiments were conducted twice with similar results.
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    ADAM9 is required for the entry phase of EMCV infection. (A) EMCV or CV-B3 were pretreated with the indicated concentrations of soluble ADAM9 protein or 100 μg/ml soluble FGFR1α/FGFR1β proteins for 1 h at 37°C and used to infect HAP1 cells. Capsid proteins (EMCV) or 3A protein (CV-B3) and nuclei (blue) were stained at 7 h postinfection. (B) HAP1 cells were pretreated with the indicated concentrations of antibody targeting ADAM9 and infected with virus for 7 h, followed by staining as described above for panel A. Representative confocal micrographs are shown in panels A, B, and C. Values (percentages) on the micrographs are mean ± SEM values for 3 or 4 (A) or 4 (B) technical replicates, normalized to the value for mock treatment. (C) WT, ADAM9 KO , or ADAM9 KO HAP1 cells overexpressing mouse ADAM9 mutant E348A (mADAM9) were incubated with EMCV or CV-B3 on ice, followed by qPCR analysis of bound virus. The values are means plus standard deviations (SD) (error bars) for three biological replicates. Experiments were conducted twice with similar results.

    Journal: mBio

    Article Title: Identification of the Cell-Surface Protease ADAM9 as an Entry Factor for Encephalomyocarditis Virus

    doi: 10.1128/mBio.01780-19

    Figure Lengend Snippet: ADAM9 is required for the entry phase of EMCV infection. (A) EMCV or CV-B3 were pretreated with the indicated concentrations of soluble ADAM9 protein or 100 μg/ml soluble FGFR1α/FGFR1β proteins for 1 h at 37°C and used to infect HAP1 cells. Capsid proteins (EMCV) or 3A protein (CV-B3) and nuclei (blue) were stained at 7 h postinfection. (B) HAP1 cells were pretreated with the indicated concentrations of antibody targeting ADAM9 and infected with virus for 7 h, followed by staining as described above for panel A. Representative confocal micrographs are shown in panels A, B, and C. Values (percentages) on the micrographs are mean ± SEM values for 3 or 4 (A) or 4 (B) technical replicates, normalized to the value for mock treatment. (C) WT, ADAM9 KO , or ADAM9 KO HAP1 cells overexpressing mouse ADAM9 mutant E348A (mADAM9) were incubated with EMCV or CV-B3 on ice, followed by qPCR analysis of bound virus. The values are means plus standard deviations (SD) (error bars) for three biological replicates. Experiments were conducted twice with similar results.

    Article Snippet: The following chemicals and reagents were used in this study: recombinant human FGFR1α IIIb (catalog no. 655-FR; R&D Systems), recombinant human FGFR1β IIIc (catalog no. 661-FR; R&D Systems), recombinant human ADAM9 (catalog no. 939-AD-020; R&D Systems), goat polyclonal anti-ADAM9 (catalog no. AF939; R&D Systems), and batimastat (catalog no. 2961; Tocris).

    Techniques: Infection, Staining, Virus, Mutagenesis, Incubation